| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | KOG1; Mip1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human RPTOR |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RPTOR抗体的3篇参考文献示例：
1. **文献名称**：*mTOR interacts with raptor to form a nutrient-sensitive complex that controls cell growth*
**作者**：Kim D.H. et al.
**摘要**：该研究首次鉴定了RPTOR(raptor)作为mTOR的关键结合蛋白，并证明其通过形成mTORC1复合体调控细胞生长。研究中利用特异性RPTOR抗体进行免疫共沉淀，揭示了其在营养感应和信号转导中的核心作用。
2. **文献名称**：*Raptor, a binding partner of target of rapamycin (mTOR), mediates mTOR function*
**作者**：Hara K. et al.
**摘要**：本文通过RPTOR抗体的免疫沉淀实验，证实了RPTOR与mTOR的相互作用，并证明其对mTOR激酶活性的调节至关重要，尤其在氨基酸信号通路中发挥关键作用。
3. **文献名称**：*The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1*
**作者**：Sancak Y. et al.
**摘要**：研究利用RPTOR抗体进行亚细胞定位分析和复合体分离，揭示了Rag GTP酶通过结合RPTOR调控mTORC1在溶酶体上的激活机制，阐明了氨基酸感知的分子基础。
这些文献均通过RPTOR抗体的实验手段(如Western blot、免疫沉淀等)，揭示了其在mTOR信号通路中的功能机制。
The RPTOR antibody is a crucial tool in studying the mechanistic target of rapamycin (mTOR) signaling pathway, which regulates cell growth, proliferation, and metabolism. RPTOR (Regulatory Associated Protein of mTOR), also known as Raptor, is a key component of the mTOR complex 1 (mTORC1). This complex integrates inputs from nutrients, growth factors, and stress signals to control anabolic processes, including protein synthesis and autophagy inhibition. Dysregulation of mTORC1 is implicated in cancers, metabolic disorders, and aging-related diseases.
RPTOR antibodies are designed to detect and quantify the expression, localization, and activity of the RPTOR protein. They are widely used in techniques such as Western blotting, immunofluorescence, immunoprecipitation, and immunohistochemistry. These antibodies help researchers investigate mTORC1 assembly, upstream/downstream signaling (e.g., interactions with Rag GTPases or substrates like S6K), and responses to inhibitors like rapamycin.
Validated RPTOR antibodies are essential for ensuring specificity, often confirmed through knockout cell lines or siRNA-mediated silencing. Commercial antibodies are typically raised in rabbits or mice, with options for monoclonal or polyclonal formats. Their applications extend to studying mTORC1's role in diseases, including tumorigenesis, insulin resistance, and neurodegeneration, making them vital for both basic research and therapeutic development targeting the mTOR pathway.
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