Mouse Fatty acid synthase(FASN) ELISA kit

产品详情

中文名： 小鼠脂肪酸合成酶(FASN)ELISA kit

货号: IPD-EL008435MO

Price： ￥2500

48T 96T

数量：

大包装询价

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：
小鼠脂肪酸合成酶(FASN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FASN含量。FASN（脂肪酸合酶）是参与内源性脂肪酸合成的关键酶。其背景在于它在肿瘤等多种疾病中异常高表达。研究机制方面，主要聚焦于它促进肿瘤细胞增殖、存活、迁移等过程，抑制 FASN 活性有望为相关疾病治疗提供新策略。试剂盒检测范围为3.12 pg/mL-200 pg/mL，该产品适用于基础科研中探究脂代谢调控机制、建立代谢性疾病动物模型或肿瘤模型后FASN的动态变化分析，以及药物干预实验中靶向FASN通路的疗效评估等研究场景，为揭示FASN在生理病理过程中的作用提供有效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
FasnFatty acid synthase ELISA Kit; EC 2.3.1.85) [Includes: [Acyl-carrier-protein] S-acetyltransferase ELISA Kit; EC 2.3.1.38); [Acyl-carrier-protein] S-malonyltransferase ELISA Kit; EC 2.3.1.39); 3-oxoacyl-[acyl-carrier-protein] synthase ELISA Kit; EC 2.3.1.41); 3-oxoacyl-[acyl-carrier-protein] reductase ELISA Kit; EC 1.1.1.100); 3-hydroxyacyl-[acyl-carrier-protein] dehydratase ELISA Kit; EC 4.2.1.59); Enoyl-[acyl-carrier-protein] reductase ELISA Kit; EC 1.3.1.39); Oleoyl-[acyl-carrier-protein] hydrolase ELISA Kit; EC 3.1.2.14)] ELISA Kit
缩写：
FASN
Uniprot No.：
P19096
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
3.12 pg/mL-200 pg/mL
灵敏度：
0.78 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Metabolism
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FASN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-97
1:200	Average %	96
	Range %	92-103
1:400	Average %	85
	Range %	81-89
1:800	Average %	89
	Range %	83-95

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
200	2.363	2.503	2.433	2.302
100	2.025	2.053	2.039	1.908
50	1.412	1.501	1.457	1.326
25	0.936	1.009	0.973	0.842
12.5	0.609	0.586	0.598	0.467
6.25	0.354	0.364	0.359	0.228
3.12	0.233	0.245	0.239	0.108
0	0.125	0.136	0.131