首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人脂肪酸合成酶(FASN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的FASN含量。FASN（脂肪酸合成酶）是一种在脂肪酸合成过程中起关键作用的酶，其在多种肿瘤细胞中表达水平升高，与肿瘤细胞的增殖、转移和抗凋亡等密切相关。研究显示，FASN通过促进脂质合成和代谢，影响肿瘤细胞的生物学行为，成为癌症治疗的潜在靶点。试剂盒检测范围为1.56 ng/mL-100 ng/mL，可广泛应用于基础科研领域，包括代谢综合征机制研究、癌症细胞脂代谢异常分析、药物干预效果评估等实验场景，为探索FASN在生理病理过程中的作用提供可靠检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：[Acyl-carrier-protein] S acetyltransferase ELISA Kit; [Acyl-carrier-protein] S malonyltransferase ELISA Kit; 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase ELISA Kit; 3-oxoacyl-[acyl-carrier-protein] reductase ELISA Kit; 3-oxoacyl-[acyl-carrier-protein] synthase ELISA Kit; Enoyl-[acyl-carrier-protein] reductase ELISA Kit; FAS ELISA Kit; FAS_HUMAN ELISA Kit; FASN ELISA Kit; Fatty acid synthase ELISA Kit; MGC14367 ELISA Kit; MGC15706 ELISA Kit; OA 519 ELISA Kit; Oleoyl-[acyl-carrier-protein] hydrolase ELISA Kit; SDR27X1 ELISA Kit; Short chain dehydrogenase/reductase family 27X member 1 ELISA Kit
缩写：FASN
Uniprot No.：P49327
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FASN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 85-96 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 92-103 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 95-108 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 86-91 | |||||
回收率：
| The recovery of human FASN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 86-95 | ||||
| EDTA plasma (n=4) | 98 | 94-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.3782.5552.4672.319501.9221.7741.8481.700251.4011.4991.4501.30212.50.9891.0591.0240.8766.250.6160.6270.6220.4743.120.3550.3440.3500.2021.560.2740.2880.2810.13300.1440.1520.148 | ||||||
货期：3-5 working days
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