首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠F-box蛋白32(FBXO32)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的FBXO32含量。FBXO32是一种重要靶点。它属于F-box蛋白家族成员，在肌肉萎缩等过程中发挥关键作用。研究显示其参与泛素-蛋白酶体途径，可识别并结合底物蛋白，促使其泛素化降解，目前在肌肉相关疾病等方面的研究机制探索备受关注。试剂盒检测范围为37.5 pg/mL-2400 pg/mL，适用于肌肉组织代谢研究、神经退行性疾病模型构建、细胞应激反应分析等科研领域，为探索FBXO32在蛋白质降解通路中的调控机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Fbxo32F-box only protein 32 ELISA kit; Atrogin-1 ELISA kit; Muscle atrophy F-box protein ELISA kit; MAFbx ELISA kit
缩写：FBXO32
Uniprot No.：Q9CPU7
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：37.5 pg/mL-2400 pg/mL
灵敏度：9.375 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse FBXO32 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 84 | |||||
| Range % | 80-92 | ||||||
| 1:2 | Average % | 96 | |||||
| Range % | 92-100 | ||||||
| 1:4 | Average % | 103 | |||||
| Range % | 99-107 | ||||||
| 1:8 | Average % | 89 | |||||
| Range % | 84-93 | ||||||
回收率：
| The recovery of mouse FBXO32 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 89-100 | |||||
| EDTA plasma (n=4) | 92 | 88-96 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected24002.8742.7582.8162.64812002.3142.2762.2952.1276001.7311.7031.7171.5493001.1051.1391.1220.9541500.6170.6020.6100.442750.3650.3810.3730.20537.50.2680.2540.2610.09300.1660.1700.168 | |||||||
货期：3-5 working days
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