首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：人纤维胶凝蛋白3(FCN3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的FCN3含量。试剂盒检测范围为7.8 ng/mL-500 ng/mL，该产品可用于体外实验中定量评估不同生理或病理条件下FCN3的表达水平，例如探索其在免疫调节、感染性疾病模型中的功能机制，或分析细胞培养体系及生物样本中的蛋白动态变化，为炎症相关信号通路、补体系统研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FCN3 ELISA Kit; FCNH ELISA Kit; HAKA1 ELISA Kit; Ficolin-3 ELISA Kit; Collagen/fibrinogen domain-containing lectin 3 p35 ELISA Kit; Collagen/fibrinogen domain-containing protein 3 ELISA Kit; Hakata antigen ELISA Kit
缩写：FCN3
Uniprot No.：O75636
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：7.8 ng/mL-500 ng/mL
灵敏度：1.95 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FCN3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 99 | |||||
| Range % | 88-104 | ||||||
| 1:200 | Average % | 101 | |||||
| Range % | 91-105 | ||||||
| 1:400 | Average % | 88 | |||||
| Range % | 83-94 | ||||||
| 1:800 | Average % | 93 | |||||
| Range % | 87-97 | ||||||
回收率：
| The recovery of human FCN3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 107 | 98-114 | |||||
| EDTA plasma (n=4) | 100 | 95-104 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected5002.8172.7212.7692.6502502.3122.2422.2772.1581251.5301.5781.5541.43562.50.7780.7640.7710.65231.20.4080.4140.4110.29215.60.2530.2430.2480.1297.80.1670.1730.1700.05100.1170.1210.119 | |||||||
货期：3-5 working days
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