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产品描述:猪血纤蛋白原降解产物(FDP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的FDP含量。FDP即1,6 - 二磷酸果糖,是糖代谢中间产物。在细胞代谢中能调节糖代谢中若干酶的活性,促进糖酵解产生ATP。为细胞供能、改善细胞代谢,在心肌缺血、休克等治疗研究中,展现出改善细胞功能、减轻损伤的潜在价值。试剂盒检测范围为1.25 μg/mL-80 μg/mL,支持科研领域对猪凝血相关疾病模型(如血栓形成、弥散性血管内凝血)、药物干预效果评估或器官损伤修复机制的研究本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
缩写:FDP
种属:Sus scrofa (Pig)
样本类型:serum, plasma, tissue homogenates
检测范围:1.25 μg/mL-80 μg/mL
灵敏度:0.31 μg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Others
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of pig FDP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 91 | ||||
Range % | 86-95 | |||||
1:2 | Average % | 97 | ||||
Range % | 92-102 | |||||
1:4 | Average % | 90 | ||||
Range % | 87-98 | |||||
1:8 | Average % | 92 | ||||
Range % | 85-96 |
回收率:
The recovery of pig FDP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 93 | 89-97 | ||||
EDTA plasma (n=4) | 98 | 95-101 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
μg/mlOD1OD2AverageCorrected801.9802.1012.0411.884401.3181.2151.2671.110200.7690.7890.7790.622100.4480.4350.4420.28550.2920.2750.2840.1272.50.2400.2510.2460.0891.250.2030.2060.2050.04800.1560.1570.157 |
货期:3-5 working days
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