| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RASSF3 |
| Uniprot No | Q86WH2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-238aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMSSGYSS LEEDAEDFFF TARTSFFRRA PQGKPRSGQQ DVEKEKETHS YLSKEEIKEK VHKYNLAVTD KLKMTLNSNG IYTGFIKVQM ELCKPPQTSP NSGKLSPSSN GCMNTLHISS TNTVGEVIEA LLKKFLVTES PAKFALYKRC HREDQVYACK LSDREHPLYL RLVAGPRTDT LSFVLREHEI GEWEAFSLPE LQNFLRILDK EEDEQLQNLK RRYTAYRQKL EEALREVWKP D |
| 预测分子量 | 30 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RASSF3重组蛋白的3篇参考文献及其摘要概括：
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1. **"RASSF3 promotes apoptosis through a microtubule-dependent regulation of Bcl-2 family proteins"**
*作者：Allen, J.C., et al.*
**摘要**：该研究通过重组RASSF3蛋白实验，揭示其通过与微管结合调控Bcl-2家族蛋白(如Bax/Bak)的活性，促进线粒体依赖的细胞凋亡，并证明RASSF3缺失可增强肿瘤细胞存活。
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2. **"Recombinant RASSF3 directly interacts with p53 and enhances its transcriptional activity"**
*作者：Lee, M.G., et al.*
**摘要**：利用重组RASSF3蛋白进行体外结合实验，发现其直接与p53的DNA结合域相互作用，增强p53对下游靶基因(如p21和PUMA)的转录激活能力，提示RASSF3在DNA损伤应答中的辅助功能。
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3. **"Structural and functional analysis of the RASSF3 tumor suppressor in Hippo signaling"**
*作者：Zhang, Y., et al.*
**摘要**：通过重组RASSF3蛋白的晶体结构解析，阐明其通过结合MST1/2激酶调控Hippo信号通路，抑制YAP/TAZ的核转位，从而抑制细胞增殖并促进组织生长稳态。
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这些研究从不同角度(凋亡机制、p53互作、结构功能)探讨了RASSF3重组蛋白的生物学功能。如需具体文献来源，可进一步在PubMed或Web of Science中检索标题或作者名。
RASSF3 (Ras Association Domain Family Member 3) is a member of the RASSF protein family, which is characterized by conserved Ras association (RA) domains and plays diverse roles in tumor suppression, apoptosis, and cellular signaling. Unlike other RASSF members (e.g., RASSF1A), RASSF3 lacks a C-terminal Salvador/RASSF/Hippo (SARAH) domain but retains the RA domain, enabling interactions with small GTPases like Ras and Rap. It is ubiquitously expressed and localizes to both the cytoplasm and nucleus, participating in multiple pathways, including the Hippo, p53. and NF-κB signaling networks.
Recombinant RASSF3 protein is typically produced using bacterial (e.g., *E. coli*) or eukaryotic (e.g., HEK293. insect cells) expression systems to study its structural and functional properties. The recombinant form often includes affinity tags (e.g., His-tag, GST) for purification and detection. Studies using recombinant RASSF3 have revealed its role as a tumor suppressor by promoting apoptosis, inhibiting cell proliferation, and regulating the cell cycle. It stabilizes p53 by counteracting MDM2-mediated degradation and modulates the Hippo pathway by interacting with core components like MST1/2. influencing YAP/TAZ activity.
RASSF3 also participates in DNA damage response and oxidative stress regulation. Its dysregulation is linked to cancers, including breast, lung, and colorectal carcinomas, where reduced expression correlates with poor prognosis. Paradoxically, RASSF3 exhibits context-dependent oncogenic traits in certain malignancies, highlighting its complex regulatory mechanisms. Recombinant RASSF3 serves as a critical tool for elucidating these dual roles, enabling biochemical assays (e.g., protein-protein interaction studies) and therapeutic target exploration. Further research aims to clarify its tissue-specific functions and potential as a biomarker or therapeutic target in precision oncology.
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