首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人纤维蛋白原β链(FGB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FGB含量。人纤维蛋白原β链（FGB）是纤维蛋白原分子的重要组成部分，由位于4号染色体上的FGB基因编码。FGB基因突变可导致纤维蛋白原结构或功能异常，引发遗传性纤维蛋白原缺陷症，表现为出血或血栓倾向。研究FGB的突变机制有助于深入了解疾病发生原理，为临床诊断和治疗提供依据。试剂盒检测范围为0.45 ng/ml-30 ng/ml，适用于生命科学研究中FGB的定量分析，例如探究凝血机制、评估炎症模型中的蛋白表达动态、或筛选影响纤维蛋白原代谢的药物靶点，为心血管疾病、肝脏功能及炎症相关基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Beta fibrinogen ELISA Kit; Epididymis Secretory Sperm Binding Protein Li 78p ELISA Kit; FGB ELISA Kit; FIBB_HUMAN ELISA Kit; Fibrinogen beta chain ELISA Kit; Fibrinogen; B Beta Polypeptide ELISA Kit; HEL-S-78p ELISA Kit
缩写：FGB
Uniprot No.：P02675
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.45 ng/ml-30 ng/ml
灵敏度：0.11 ng/ml
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FPB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 91 | |
| Range % | 85-96 | ||
| 1:2 | Average % | 92 | |
| Range % | 87-100 | ||
| 1:4 | Average % | 91 | |
| Range % | 86-98 | ||
| 1:8 | Average % | 95 | |
| Range % | 87-105 |
回收率：
| The recovery of human FPB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 90-103 | ||||
| EDTA plasma (n=4) | 90 | 87-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 302.3172.4252.3712.243 151.5751.6011.5881.460 7.50.9060.9370.9220.794 3.750.5520.5680.5600.432 1.80.3740.3610.3680.240 0.90.2590.2650.2620.134 0.450.1740.1780.1760.048 00.1270.1290.128 | ||||||
货期：3-5 working days
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