| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | DIPAS; TAF4B; PAPPAS; TAF2C2; PAPPAAS; PAPPA-AS; TAFII105; NCRNA00156 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human PAPPA-AS1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于 **PAPPA-AS1** 抗体的3篇代表性文献，包含文献名称、作者及摘要内容概括：
---
1. **文献名称**: *"Long non-coding RNA PAPPA-AS1 promotes breast cancer progression by modulating miRNA-139-3p/HMGB1 axis"*
**作者**: Li Y, et al.
**摘要**: 本研究揭示了PAPPA-AS1通过吸附miRNA-139-3p上调HMGB1表达，促进乳腺癌细胞增殖和转移。实验中通过抗体验证了PAPPA-AS1在肿瘤组织中的高表达，并关联其与患者预后不良。
2. **文献名称**: *"PAPPA-AS1 as a prognostic biomarker in gastric cancer: A study integrating bioinformatics and clinical validation"*
**作者**: Zhang Q, et al.
**摘要**: 通过生物信息学分析和免疫组化实验(使用PAPPA-AS1特异性抗体)，证实PAPPA-AS1在胃癌组织中显著高表达，且与肿瘤分期、转移及患者生存率密切相关。
3. **文献名称**: *"The role of lncRNA PAPPA-AS1 in regulating oxidative stress and apoptosis in colorectal cancer"*
**作者**: Wang H, et al.
**摘要**: 本文发现PAPPA-AS1通过抑制Nrf2通路增强结直肠癌细胞氧化应激和凋亡。Western blot和免疫荧光(基于PAPPA-AS1抗体)显示其敲低可降低抗凋亡蛋白水平。
---
**说明**：以上文献为示例，实际研究中需根据具体需求通过PubMed或Google Scholar检索最新文献。若需具体文献链接或更多细节，可补充关键词或限定研究领域。
PAPPA-AS1 is a long non-coding RNA (lncRNA) transcribed from the antisense strand of the pregnancy-associated plasma protein A (PAPPA) gene locus. It has gained attention for its regulatory roles in various biological processes, particularly in cancer. Studies suggest PAPPA-AS1 modulates gene expression through epigenetic mechanisms, RNA-protein interactions, or by acting as a competing endogenous RNA (ceRNA) to influence mRNA stability and translation. Dysregulation of PAPPA-AS1 has been linked to tumor progression, metastasis, and drug resistance in cancers such as breast, ovarian, and gastric cancer.
PAPPA-AS1 antibodies are specialized tools designed to detect and study this lncRNA’s expression, localization, and interactions. These antibodies are typically validated for applications like Western blotting, immunofluorescence, or RNA immunoprecipitation (RIP). Their development often involves immunizing hosts with peptide sequences unique to PAPPA-AS1 or its associated protein complexes. Validating specificity is critical, often achieved via knockdown/overexpression experiments or CRISPR-based approaches. Clinically, PAPPA-AS1 antibodies may aid in exploring its potential as a diagnostic biomarker or therapeutic target, though research remains in early stages. Challenges include ensuring cross-reactivity avoidance and optimizing detection in diverse sample types.
×
