首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人碱性成纤维细胞生长因子(bFGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FGF2含量。FGF2即成纤维细胞生长因子2，是一种多功能细胞因子。它在胚胎发育、血管生成、组织修复等生理过程中发挥重要作用。研究多聚焦于其信号通路，如与受体结合激活下游信号分子，影响细胞增殖、分化等，是生物医学领域颇具潜力的研究靶点。试剂盒检测范围为1.56 pg/mL-100 pg/mL，适用于体外培养细胞上清、动物模型组织或体液样本的检测；科研人员可通过该产品探究bFGF在细胞信号通路调控、组织再生、肿瘤微环境中的作用，或用于评估药物干预、基因编辑等实验条件下bFGF的表达变化；严格适用于生命科学基础研究，为探索bFGF相关分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Basic fibroblast growth factor ELISA Kit; Basic fibroblast growth factor bFGF ELISA Kit; BFGF ELISA Kit; FGF 2 ELISA Kit; FGF B ELISA Kit; FGF-2 ELISA Kit; Fgf2 ELISA Kit; FGF2 basic ELISA Kit; FGF2_HUMAN ELISA Kit; FGFB ELISA Kit; Fibroblast growth factor 2 (basic) ELISA Kit; Fibroblast growth factor 2 ELISA Kit; Fibroblast growth factor; basic ELISA Kit; HBGF 2 ELISA Kit; HBGF-2 ELISA Kit; HBGF2 ELISA Kit; HBGH 2 ELISA Kit; HBGH2 ELISA Kit; Heparin binding growth factor 2 precursor ELISA Kit; Heparin-binding growth factor 2 ELISA Kit; Prostatropin ELISA Kit
缩写：FGF2
Uniprot No.：P09038
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human bFGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 93-103 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human bFGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 85-98 | ||||
| EDTA plasma (n=4) | 92 | 89-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 1002.5342.3562.4452.349 501.9611.9571.9591.863 251.5451.5891.5671.471 12.51.1571.1691.1631.067 6.250.7180.7460.7320.636 3.120.4470.4860.4670.371 1.560.2310.2410.2360.140 00.0980.0940.096 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human bFGF antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled bFGF antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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