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产品描述：人成纤维细胞生长因子21 (FGF21/UNQ3115/PRO10196)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的FGF21含量。FGF21 是一种成纤维细胞生长因子。其背景上，它在能量代谢、糖脂代谢等方面有重要调节作用。研究机制方面，主要是通过与特定受体结合，激活下游信号通路，进而参与调节代谢相关基因表达，有望成为治疗代谢性疾病的新靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于代谢相关疾病的分子机制研究、药物开发中生物标志物检测，以及体外培养系统中FGF21分泌水平的动态监测，为生物医学研究提供标准化工具。支持科研人员探究FGF21在细胞模型、动物实验或临床前样本中的表达调控机制。适用于分析血清、血浆、细胞培养上清及组织匀浆样本中FGF21的蛋白水平。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FGF 21 ELISA Kit; FGF-21 ELISA Kit; Fgf21 ELISA Kit; FGF21_HUMAN ELISA Kit; FGFL ELISA Kit; Fibroblast growth factor 21 ELISA Kit; PRO10196 ELISA Kit; UNQ3115 ELISA Kit
缩写：FGF21
Uniprot No.：Q9NSA1
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FGF21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-99 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 84-96 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human FGF21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-105 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10001.9892.0362.0131.8115001.6211.6411.6311.4292501.1161.1321.1240.9221250.6820.6980.6900.48862.50.4980.5060.5020.30031.20.3750.3890.3820.18015.60.2860.2990.2930.09100.2030.2010.202 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human FGF21 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled FGF21 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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