首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人成纤维细胞生长因子23(FGF-23)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织培养上清液、尿液、组织匀浆样本中的FGF23含量。FGF23是重要的靶点。它是成纤维细胞生长因子家族成员，主要由骨细胞和骨细胞分泌，参与调节体内磷和维生素D代谢。研究机制多聚焦于其信号通路，以及在慢性肾脏疾病、遗传性低磷血症等相关疾病中的作用机制，为治疗提供方向。试剂盒检测范围为3.12 pg/mL-200 pg/mL，本产品可广泛应用于基础医学研究，例如探究慢性肾病进程中矿物质代谢失衡机制、骨骼发育相关信号通路调控，或评估药物干预对FGF - 23表达水平的影响，亦可用于生物样本库中FGF - 23生物标志物的系统性筛查，为代谢性疾病研究提供可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ADHR ELISA Kit; FGF-23 ELISA Kit; Fgf23 ELISA Kit; FGF23_HUMAN ELISA Kit; FGFN ELISA Kit; Fibroblast growth factor 23 ELISA Kit; Fibroblast growth factor 23 C-terminal peptide ELISA Kit; Fibroblast growth factor 23 precursor ELISA Kit; HPDR2 ELISA Kit; HYPF ELISA Kit; Phosphatonin ELISA Kit; PHPTC ELISA Kit; Tumor derived hypophosphatemia inducing factor ELISA Kit; Tumor-derived hypophosphatemia-inducing factor ELISA Kit
缩写：FGF23
Uniprot No.：Q9GZV9
种属：Homo sapiens (Human)
样本类型：serum, cell culture supernates, urine, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FGF-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 99 | ||||
| Range % | 80-115 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 91-110 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-115 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-100 | |||||
回收率：
| The recovery of human FGF-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.9582.9862.9722.7831002.4612.4752.4682.279501.8521.8891.8711.682251.2341.2861.2601.07112.50.7530.7860.7700.5816.250.5310.5560.5440.3553.120.3960.4160.4060.21700.1920.1860.189 | ||||||
货期：3-5 working days
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