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产品描述：牛成纤维细胞生长因子23(FGF23)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的FGF23含量。FGF23即成纤维细胞生长因子 23，主要由骨细胞分泌。它能调节磷代谢和维生素 D 水平，与多种疾病相关，如慢性肾脏病矿物质和骨异常。研究机制集中于其信号通路，包括与 klotho 蛋白相互作用及下游信号传导等。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于科研领域探究牛磷代谢机制、骨骼疾病模型构建、肾脏功能研究及相关营养调控实验，为畜牧兽医研究、动物营养学及基础医学领域提供可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：FGF23
Uniprot No.：E1BJU2
种属：Bos taurus (Bovine)
样本类型：serum, plasma
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays):CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine FGF23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 95 | |||||
| Range % | 86-99 | ||||||
| 1:2 | Average % | 103 | |||||
| Range % | 97-107 | ||||||
| 1:4 | Average % | 91 | |||||
| Range % | 81-94 | ||||||
| 1:8 | Average % | 100 | |||||
| Range % | 90-105 | ||||||
回收率：
| The recovery of bovine FGF23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 104 | 94-108 | |||||
| EDTA plasma (n=4) | 89 | 84-94 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 10001.9281.9521.9401.809 5001.4021.3241.3631.232 2500.8780.8620.8700.739 1250.5530.5400.5470.416 62.50.3290.3410.3350.204 31.20.2410.2560.2490.118 15.60.1780.1740.1760.045 00.1290.1330.131 | |||||||
货期：3-5 working days
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