首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠成纤维细胞生长因子23(FGF23)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FGF23含量。FGF23 是成纤维细胞生长因子 23，在调节磷和维生素 D 代谢中起关键作用。其异常表达与多种疾病相关，如慢性肾脏病矿物质和骨异常。研究机制主要聚焦于它与受体结合，调控下游信号通路，影响磷重吸收和维生素 D 活化。试剂盒检测范围为23.44 pg/mL-1500 pg/mL，适用于科研领域对FGF23蛋白水平的精确分析；为研究骨骼发育、矿物质代谢紊乱、肾脏疾病等动物模型的分子机制提供可靠工具；满足细胞实验、疾病模型评估及药物干预研究等科研需求；支持科研人员高效完成样本中FGF23的定量检测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Fgf23Fibroblast growth factor 23 ELISA kit; FGF-23 ELISA kit
缩写：FGF23
Uniprot No.：Q9EPC2
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：23.44 pg/mL-1500 pg/mL
灵敏度：5.86 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse FGF23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 102 | |||||
| Range % | 98-107 | ||||||
| 1:2 | Average % | 85 | |||||
| Range % | 80-90 | ||||||
| 1:4 | Average % | 91 | |||||
| Range % | 85-95 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 90-98 | ||||||
回收率：
| The recovery of mouse FGF23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 85-94 | |||||
| EDTA plasma (n=4) | 97 | 93-101 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected15002.6142.4682.5412.3867502.1032.0952.0991.9443751.5871.5131.5501.395187.51.0021.0741.0380.88393.750.5640.5540.5590.40446.880.3560.3820.3690.21423.440.2450.2390.2420.08700.1590.1510.155 | |||||||
货期：3-5 working days
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