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产品描述：人成纤维细胞生长因子5(FGF5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FGF5含量。FGF5（成纤维细胞生长因子5）是一种分泌信号蛋白，主要在间充质和骨骼肌中表达。它对哺乳动物毛发周期性生长起关键作用，同时与动物肢体发育及人类多形性胶质母细胞瘤、黑色素瘤、肝细胞癌和高血压有关。研究显示，FGF5通过调节细胞增殖和信号通路影响这些过程。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外研究场景，如探究FGF5在毛囊生物学、皮肤病理模型或组织工程中的功能机制，亦可应用于药物开发中靶向分子效应的评估。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FGF 5 ELISA Kit; FGF-5 ELISA Kit; FGF5 ELISA Kit; FGF5_HUMAN ELISA Kit; Fibroblast growth factor 5 ELISA Kit; HBGF 5 ELISA Kit; HBGF-5 ELISA Kit; heparin binding growth factor 5 ELISA Kit; Heparin-binding growth factor 5 ELISA Kit; Smag 82 ELISA Kit; Smag-82 ELISA Kit; TCMGLY ELISA Kit
缩写：FGF5
Uniprot No.：P12034
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FGF5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 85-93 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:8 | Average % | 106 | ||||
| Range % | 100-113 | |||||
回收率：
| The recovery of human FGF5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 91 | 86-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.3142.2272.2712.1265001.8161.8761.8461.7012501.3341.4021.3681.2231250.8010.8400.8210.67662.50.5440.5180.5310.38631.20.3720.3590.3660.22115.60.2710.2820.2770.13200.1420.1470.145 | ||||||
货期：3-5 working days
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