首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人成纤维细胞生长因子8(FGF8)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的FGF8含量。FGF8是成纤维细胞生长因子家族成员，在胚胎发育中起关键作用，调控骨骼、神经和生殖系统等发育。其表达异常可能导致骨骼稳态紊乱和发育畸形。研究机制主要涉及FGF8与FGFR结合调控细胞活动，以及其在特定组织中的表达和功能。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，本试剂盒广泛应用于基础研究领域，包括发育生物学中胚胎形态发生机制探索、肿瘤微环境中生长因子信号通路研究，以及神经退行性疾病相关分子调控网络分析等方向，为体外实验提供高效的FGF8蛋白定量解决方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AIGF ELISA Kit; Androgen induced growth factor ELISA Kit; Androgen-induced growth factor ELISA Kit; FGF 8 ELISA Kit; FGF-8 ELISA Kit; FGF-8b ELISA Kit; FGF8 ELISA Kit; FGF8_HUMAN ELISA Kit; Fibroblast growth factor 8 (androgen induced) ELISA Kit; Fibroblast growth factor 8 ELISA Kit; Fibroblast growth factor 8 precursor ELISA Kit; HBGF 8 ELISA Kit; HBGF-8 ELISA Kit; HBGF8 ELISA Kit; Heparin-binding growth factor 8 ELISA Kit; HH6 ELISA Kit; KAL6 ELISA Kit
缩写：FGF8
Uniprot No.：P55075
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FGF8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human FGF8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.4552.4982.4772.2905001.8721.9321.9021.7152501.2131.2361.2251.0381250.6870.6990.6930.50662.50.4990.4840.4920.30531.20.3790.3980.3890.20215.60.2770.2870.2820.09500.1880.1860.187 | ||||||
货期：3-5 working days
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