首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人成纤维细胞生长因子受体3(FGFR3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FGFR3含量。FGFR3 是一个重要的靶点。它属于成纤维细胞生长因子受体家族，在细胞增殖、分化等过程中发挥关键作用。研究发现其异常激活或突变与多种肿瘤的发生发展相关，通过抑制 FGFR3 的活性来阻断相关信号通路，成为肿瘤治疗的潜在策略。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于基础医学研究中FGFR3在细胞信号通路、疾病模型动物体液样本或组织特异性表达分析等场景，例如探究FGFR3在特定病理条件下的表达调控机制、药物干预效果评估或相关分子互作研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ACH ELISA Kit; CD 333 ELISA Kit; CD333 ELISA Kit; CD333 antigen ELISA Kit; CEK 2 ELISA Kit; CEK2 ELISA Kit; FGFR 3 ELISA Kit; FGFR-3 ELISA Kit; FGFR3 ELISA Kit; FGFR3_HUMAN ELISA Kit; Fibroblast growth factor receptor 3 (achondroplasia thanatophoric dwarfism) ELISA Kit; Fibroblast growth factor receptor 3 ELISA Kit; Heparin binding growth factor receptor ELISA Kit; HSFGFR3EX ELISA Kit; Hydroxyaryl protein kinase ELISA Kit; JTK 4 ELISA Kit; JTK4 ELISA Kit; MFR 3 ELISA Kit; SAM 3 ELISA Kit; Tyrosine kinase JTK 4 ELISA Kit; Tyrosine kinase JTK4 ELISA Kit; Z FGFR 3 ELISA Kit
缩写：FGFR3
Uniprot No.：P22607
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FGFR3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-92 | |||||
| 1:2 | Average % | 86 | ||||
| Range % | 83-89 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:8 | Average % | 104 | ||||
| Range % | 97-108 | |||||
回收率：
| The recovery of human FGFR3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 82-94 | ||||
| EDTA plasma (n=4) | 86 | 80-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.7452.8682.8072.70320002.2872.1822.2352.13110001.7161.6161.6661.5625000.9430.9630.9530.8492500.5470.5220.5350.4311250.3440.3610.3530.24962.50.2460.2570.2520.14800.1030.1050.104 | ||||||
货期：3-5 working days
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