首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠纤维连接蛋白Ⅲ型结构域蛋白5(FNDC5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FNDC5含量。FNDC5即纤维连接蛋白结构域蛋白5，是一种跨膜蛋白。其剪切产物鸢尾素具多种生理功能。研究机制方面，它参与能量代谢调节，能增加脂肪氧化和能量消耗，还对骨骼、肌肉等组织的生长和修复有影响，在代谢疾病防治等领域受关注。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于科研领域对FNDC5表达水平的分析；尤其适用于探究运动干预、代谢调控机制、肌肉生理学及神经退行性疾病模型中的FNDC5动态变化；为代谢研究、运动生理学及分子机制探索提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Fndc5Fibronectin type III domain-containing protein 5 [Cleaved into: Irisin] ELISA kit
缩写：FNDC5
Uniprot No.：Q8K3V5
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat FNDC5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 98-109 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rat FNDC5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-99 | ||||
| EDTA plasma (n=4) | 89 | 84-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 402.5482.4522.5002.308 201.8761.9311.9041.712 101.2511.3141.2831.091 50.7800.7920.7860.594 2.50.4970.4830.4900.298 1.250.4020.3850.3940.202 0.6250.2730.2790.2760.084 00.1950.1880.192 | ||||||
货期：3-5 working days
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