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产品描述：人谷氨酸羧肽酶2(FOLH1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FOLH1含量。FOLH1 是一种重要的靶点。它在多种生理和病理过程中发挥作用，尤其在前列腺癌等肿瘤组织中高表达。研究机制方面，主要聚焦于其参与的叶酸代谢途径，通过阻断该靶点可能抑制肿瘤细胞增殖，是癌症治疗药物研发的热门方向。试剂盒检测范围为4.7 ng/mL-300 ng/mL，该产品适用于生命科学领域对FOLH1蛋白表达量的研究，例如肿瘤微环境分析、代谢性疾病模型构建或神经生物学相关机制探索，可为体外实验提供细胞、动物或人源样本的定量检测支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cell growth inhibiting protein 27 ELISA Kit; Cell growth-inhibiting gene 27 protein ELISA Kit; FGCP ELISA Kit; Folate hydrolase (prostate-specific membrane antigen) 1 ELISA Kit; Folate hydrolase 1 ELISA Kit; Folate hydrolase ELISA Kit; Folate hydrolase prostate specific membrane antigen 1 ELISA Kit; FOLH 1 ELISA Kit; FOLH ELISA Kit; Folh1 ELISA Kit; FOLH1_HUMAN ELISA Kit; Folylpoly gamma glutamate carboxypeptidase ELISA Kit; Folylpoly-gamma-glutamate carboxypeptidase ELISA Kit; GCP 2 ELISA Kit; GCP II ELISA Kit; GCP2 ELISA Kit; GCPII ELISA Kit; GIG27 ELISA Kit; Glutamate carboxylase II ELISA Kit; Glutamate carboxypeptidase 2 ELISA Kit; Glutamate carboxypeptidase II ELISA Kit; Membrane glutamate carboxypeptidase ELISA Kit; mGCP ELISA Kit; N acetylated alpha linked acidic dipeptidase 1 ELISA Kit; N-acetylated-alpha-linked acidic dipeptidase I ELISA Kit; NAALAD 1 ELISA Kit; NAALAD1 ELISA Kit; NAALAdase ELISA Kit; NAALADase I ELISA Kit; Prostate specific membrane antigen ELISA Kit; Prostate specific membrane antigen variant F ELISA Kit; Prostate-specific membrane antigen ELISA Kit; PSM ELISA Kit; PSMA ELISA Kit; Pteroylpoly gamma glutamate carboxypeptidase ELISA Kit; Pteroylpoly-gamma-glutamate carboxypeptidase ELISA Kit
缩写：FOLH1
Uniprot No.：Q04609
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：4.7 ng/mL-300 ng/mL
灵敏度：1.2 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FOLH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 92 | ||||
| Range % | 85-98 | |||||
| 1:10 | Average % | 95 | ||||
| Range % | 85-100 | |||||
| 1:20 | Average % | 96 | ||||
| Range % | 89-103 | |||||
| 1:40 | Average % | 89 | ||||
| Range % | 81-98 | |||||
回收率：
| The recovery of human FOLH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 89-105 | ||||
| EDTA plasma (n=4) | 96 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 3002.8712.8892.8802.776 1502.5292.5322.5312.427 751.8821.8971.8901.786 37.51.1741.1651.1701.066 18.80.6990.7060.7030.599 9.40.4820.5010.4920.388 4.70.3080.3150.3120.208 00.1060.1020.104 | ||||||
货期：3-5 working days
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