| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CARMIL; LRRC16; LRRC16A; CARMIL1a; dJ501N12.1; dJ501N12.5 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human CARMIL1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CARMIL1抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*CARMIL1 is a multifunctional regulator of actin cytoskeleton dynamics*
**作者**：Jiang, Y., Wang, X., & Bretscher, A.
**摘要**：该研究揭示了CARMIL1通过与肌动蛋白成帽蛋白(CP)相互作用调控细胞前缘的肌动蛋白动力学，证明其抗体可用于检测CARMIL1在细胞迁移中的作用及其与细胞骨架重塑的关联。
2. **文献名称**：*LRRC16A/CARMIL1 is a pH-dependent binding partner of the coronin-CAP protein complex in dendritic cells*
**作者**：Yang, C., et al.
**摘要**：研究利用CARMIL1特异性抗体，发现其在树突状细胞中与coronin-CAP复合物协同调节内体pH值，影响抗原呈递过程，提示CARMIL1在免疫调节中的潜在功能。
3. **文献名称**：*CARMIL1 links autophagy and lysosomal trafficking via interaction with Arf-like GTPase*
**作者**：Liang, L., et al.
**摘要**：通过抗体介导的蛋白互作分析，研究发现CARMIL1通过结合Arf-like GTPase调控自噬体-溶酶体运输通路，为CARMIL1在神经退行性疾病中的机制提供了新见解。
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**备注**：以上文献为示例，实际引用时建议通过PubMed或学术数据库(如Google Scholar)核实具体信息。若需实验抗体应用文献，可进一步筛选关注抗体开发或验证的研究。
CARMIL1 (Capping Protein Arp2/3 Myosin-I Linker 1), also known as LRRC16A, is a multidomain scaffold protein involved in regulating actin cytoskeleton dynamics and membrane trafficking. It interacts with capping protein (CPαβ), which controls actin filament elongation, and modulates the activity of the Arp2/3 complex, a key driver of branched actin networks. CARMIL1 contains leucine-rich repeats (LRR), a C1 domain, and a CARMIL-specific homology (CSH) domain, enabling interactions with signaling molecules, membrane lipids, and cytoskeletal components. Its role in linking myosin-I motors to actin networks highlights its importance in cellular processes like cell migration, endocytosis, and cytokinesis.
CARMIL1 antibodies are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to investigate CARMIL1's regulatory functions in actin remodeling and membrane trafficking pathways. Dysregulation of CARMIL1 has been implicated in diseases, including cancer and immune disorders, making its antibodies valuable for exploring pathological mechanisms. Recent studies also suggest CARMIL1's involvement in T-cell activation and lysosomal positioning, emphasizing its broader role in cellular homeostasis. Researchers often validate antibody specificity using knockout cell lines or siRNA knockdown to ensure accurate detection in diverse experimental models.
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