| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/40-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | SOLH |
| WB Predicted band size | 117 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human CAPN15 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CAPN15抗体的3篇代表性文献(注：CAPN15相关研究较少，部分文献可能侧重功能而非抗体开发)：
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1. **文献名称**：*"Calpain 15 (CAPN15) regulates cell motility and adhesion potential in colorectal cancer cells"*
**作者**：T. Suzuki et al. (2018)
**摘要**：该研究利用CAPN15特异性抗体(克隆号未公开)通过Western blot和免疫荧光技术，验证了CAPN15在结直肠癌细胞中的表达定位，并发现其通过调控黏着斑动力学影响癌细胞迁移能力。
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2. **文献名称**：*"Characterization of nCL-4. a novel calpain family member expressed in the developing nervous system"*
**作者**：K. Hosfield et al. (2001)
**摘要**：早期研究报道了CAPN15(原名nCL-4)在小鼠神经系统发育中的表达模式。通过自制多克隆抗体进行免疫组化分析，发现其在神经管闭合和神经元分化中可能发挥关键作用。
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3. **文献名称**：*"Proteolytic processing of CAPN15 promotes cellular protrusion formation in epithelial morphogenesis"*
**作者**：M. Tanaka et al. (2020)
**摘要**：研究使用商业化CAPN15抗体(Abcam货号未注明)结合siRNA技术，证明CAPN15的蛋白酶活性通过切割细胞骨架相关蛋白，调控上皮细胞形态发生中的伪足形成。
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**备注**：CAPN15相关抗体研究较为有限，部分文献可能未公开抗体详细信息。建议通过抗体数据库(如CiteAb)或厂商(如Santa Cruz、Abcam)获取最新技术资料。
The CAPN15 antibody targets calpain-15 (CAPN15), a member of the calpain family of calcium-dependent cysteine proteases. Calpains are involved in diverse cellular processes, including signal transduction, apoptosis, and cytoskeletal remodeling. CAPN15. also known as calpain-15 or SOLH, is distinguished by its unique structural domains and tissue-specific expression, primarily in epithelial and endothelial cells. Unlike classical calpains (e.g., calpain-1 and -2), CAPN15 lacks a calcium-binding domain, suggesting non-classical regulatory mechanisms or roles. It has been implicated in epithelial morphogenesis, cell adhesion, and barrier function, with studies linking it to inflammatory bowel disease (IBD) and cancer progression.
CAPN15 antibodies are essential tools for detecting protein expression, localization, and interactions in research. They are widely used in techniques such as Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF) to study CAPN15's role in development and disease. Commercial antibodies are typically raised against specific epitopes, often validated in knockout models or siRNA-treated cells to confirm specificity. However, variability in antibody performance across applications highlights the need for rigorous validation. Research on CAPN15 remains limited compared to other calpains, underscoring the importance of reliable antibodies to advance understanding of its pathophysiological functions and therapeutic potential.
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