| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CANP10; NIDDM1 |
| WB Predicted band size | 75 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human CAPN10 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CAPN10抗体的3篇参考文献示例(注：以下文献信息为示例性虚构，仅作格式参考，实际引用需核实真实文献)：
1. **文献名称**: *CAPN10 Antibody Characterization and Its Role in Type 2 Diabetes Research*
**作者**: Smith A, et al.
**摘要**: 本研究利用特异性CAPN10抗体，通过Western blot和免疫组化技术，验证了CAPN10蛋白在胰岛β细胞中的表达，并发现其在胰岛素分泌调控中的潜在作用，为2型糖尿病发病机制提供了新见解。
2. **文献名称**: *Tissue-Specific Expression of CAPN10 Detected by Novel Monoclonal Antibodies*
**作者**: Johnson R, et al.
**摘要**: 开发了一种高特异性CAPN10单克隆抗体，用于检测人类肝脏、脂肪及肌肉组织中CAPN10的差异表达，结果表明其在代谢活跃组织中表达显著升高，提示其与能量代谢的关联。
3. **文献名称**: *CAPN10 Antibody Application in Cellular Apoptosis Studies*
**作者**: Lee H, et al.
**摘要**: 通过免疫荧光和流式细胞术，研究发现CAPN10抗体标记的蛋白在氧化应激诱导的细胞凋亡过程中表达上调，提示CAPN10可能在细胞应激反应中发挥调控功能。
如需真实文献，建议通过PubMed或Google Scholar检索关键词“CAPN10 antibody”、“CAPN10 immunoblotting”或结合具体研究领域(如糖尿病、代谢综合征)。
Calpain 10 (CAPN10) is a member of the calpain family of calcium-dependent cysteine proteases, which play roles in intracellular signaling, apoptosis, and glucose metabolism. Discovered through genome-wide association studies, CAPN10 gained attention due to its link to type 2 diabetes mellitus (T2DM). Specific polymorphisms, such as SNP-43. in the CAPN10 gene are associated with increased susceptibility to T2DM, insulin resistance, and metabolic disorders, though its exact mechanisms remain under investigation. CAPN10 antibodies are essential tools for studying the protein’s expression, localization, and function in these contexts.
These antibodies are typically developed using immunogenic peptides or recombinant CAPN10 proteins and are validated for applications like Western blotting, immunohistochemistry, and ELISA. Researchers use them to explore CAPN10’s tissue-specific expression, interaction with other proteins (e.g., calpastatin), and role in cellular processes like mitochondrial function or insulin secretion. Commercial CAPN10 antibodies are available as monoclonal or polyclonal variants from suppliers like Santa Cruz Biotechnology and Abcam.
Studies employing CAPN10 antibodies have provided insights into its paradoxical roles—both protective and detrimental—in metabolic diseases. For example, altered CAPN10 levels correlate with β-cell dysfunction and adipose tissue inflammation, highlighting its potential as a therapeutic target. However, variability in antibody specificity and cross-reactivity with other calpain isoforms (e.g., CAPN1/2) necessitates careful experimental validation to ensure data reliability. Overall, CAPN10 antibodies remain critical for unraveling the protein’s contribution to diabetes pathogenesis and metabolic dysregulation.
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