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产品描述：犬促卵泡素(FSH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FSH含量。FSH即促卵泡生成素，是腺垂体分泌的糖蛋白激素，对生殖系统发育和功能维持至关重要。其研究机制主要围绕调节卵泡发育、精子生成等生殖过程，还涉及与下丘脑-垂体-性腺轴的相互作用，对不孕不育等疾病的治疗有重要意义。试剂盒检测范围为0.35 mIU/mL-140 mIU/mL，科研人员可将其应用于犬类生殖生理学研究、繁殖障碍机制探索、生殖相关药物或营养干预效果评估等领域，例如通过检测发情周期、卵巢功能异常或睾丸发育相关样本中的FSH动态变化，为犬科动物繁殖生物学研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：FSH
Uniprot No.：E2RJZ8
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：0.35 mIU/mL-140 mIU/mL
灵敏度：0.35 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of canine FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 94-106 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-105 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 93-104 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 85-99 | |||||
回收率：
| The recovery of canine FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-99 | ||||
| EDTA plasma (n=4) | 96 | 92-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2Average1400.4390.4440.44226.250.7340.7280.7314.3751.0661.0681.0671.2251.2891.2931.2910.351.5751.5811.57802.5172.5222.520 | ||||||
货期：3-5 working days
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