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产品描述：牛促卵泡素(FSH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FSH含量。FSH（促卵泡激素）是一种由垂体前叶分泌的激素，主要作用是调节性腺活动，如促进女性卵泡生长和男性精子生成。其作用机制是通过与靶细胞表面的FSH受体结合，引发细胞内信号传导，进而调节相关基因的表达。FSH在生殖、生长发育和代谢等方面都发挥着重要作用。试剂盒检测范围为2 mIU/mL-800 mIU/mL，可用于繁殖生物学研究、生殖疾病机制探索、促排卵药物效果评估；支持从血液循环系统到性腺组织微环境的系统性分析，如卵巢卵泡发育动态监测、睾丸功能评估或垂体 - 性腺轴调控机制研究；为畜牧繁殖优化、生殖内分泌学基础研究及兽药开发等科研领域提供精准数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FSHB ELISA Kit; Follitropin subunit beta ELISA Kit; Follicle-stimulating hormone beta subunit ELISA Kit; FSH-B ELISA Kit; FSH-beta ELISA Kit; Follitropin beta chain ELISA Kit
缩写：FSH
Uniprot No.：P04837
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：2 mIU/mL-800 mIU/mL
灵敏度：2 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 91-105 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 89-100 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-100 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-107 | |||||
回收率：
| The recovery of bovine FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 89-96 | ||||
| EDTA plasma (n=4) | 94 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2Average 8000.4480.4390.444 1500.8210.8240.823 251.3541.3291.342 71.7111.7051.708 22.0192.0112.015 02.5552.5662.561 | ||||||
货期：3-5 working days
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