首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人分泌卷曲相关蛋白3(FRZB)酶联免疫试剂盒双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FRZB含量。FRZB作为一个靶点，其背景在于它在相关生理或病理过程中有重要作用。研究机制上，可能参与调控特定信号通路，影响细胞的增殖、分化等。对其深入研究有助于了解疾病发生发展机制，为开发针对FRZB的治疗方法和药物提供理论基础。试剂盒检测范围为0.47 ng/mL-30 ng/mL，适用于骨代谢研究、关节疾病机制探索、肿瘤微环境分析以及相关分子信号通路的基础研究，为体外实验、动物模型或疾病关联性研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FRZB ELISA Kit; FIZ ELISA Kit; FRE ELISA Kit; FRP ELISA Kit; FRZB1 ELISA Kit; SFRP3 ELISA Kit; Secreted frizzled-related protein 3 ELISA Kit; sFRP-3 ELISA Kit; Frezzled ELISA Kit; Fritz ELISA Kit; Frizzled-related protein 1 ELISA Kit; FrzB-1 ELISA Kit
缩写：FRZB
Uniprot No.：Q92765
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.47 ng/mL-30 ng/mL
灵敏度：0.117 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FRZB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 91 | |||||
| Range % | 85-95 | ||||||
| 1:2 | Average % | 88 | |||||
| Range % | 81-92 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 87-99 | ||||||
| 1:8 | Average % | 104 | |||||
| Range % | 97-108 | ||||||
回收率：
| The recovery of human FRZB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 84-95 | |||||
| EDTA plasma (n=4) | 93 | 87-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected302.0591.9882.0241.868151.6661.5781.6221.4667.51.1751.2081.1921.0363.750.7660.7340.7500.5941.870.5590.5450.5520.3960.940.3930.3810.3870.2310.470.2820.2760.2790.12300.1590.1530.156 | |||||||
货期：3-5 working days
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