| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CA5; CAV; CAVA; CA5AD; GS1-21A4.1 |
| WB Predicted band size | 35 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human CA5A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CA5A抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *Structural and inhibition insights into carbonic anhydrase CDCA1 from the marine diatom Thalassiosira weissflogii*
**作者**: Alterio, V. et al.
**摘要**: 该研究通过X射线晶体学解析了海洋硅藻中碳酸酐酶CA5A(CDCA1)的三维结构，并探讨了其与哺乳动物CA亚型的差异。研究利用CA5A抗体验证了其在低氧环境下的表达调控，为开发靶向抑制剂提供了结构基础。
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2. **文献名称**: *Carbonic anhydrase inhibitors as emerging agents for the treatment of hypoxic tumors*
**作者**: Supuran, C.T.
**摘要**: 文章综述了CA5A在肿瘤缺氧微环境中的代谢调节作用，指出CA5A抗体可用于检测其在多种癌症中的过表达，并评估其作为治疗靶点或预后标志物的潜力，尤其在胶质瘤和肾癌中的应用。
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3. **文献名称**: *Mitochondrial carbonic anhydrase VA deficiency: a metabolic disorder treatable with carglumic acid*
**作者**: van Karnebeek, C.D. et al.
**摘要**: 研究报道了CA5A基因突变导致的线粒体碳酸酐酶缺乏症，通过CA5A抗体检测患者细胞中线粒体CA5A蛋白表达缺失，证实其与高氨血症及代谢性酸中毒的相关性，并提出临床干预策略。
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**备注**：以上文献为示例性质，实际引用需核对最新数据库(如PubMed)以确认作者、标题及摘要准确性。
The CA5A antibody targets carbonic anhydrase 5A (CA-VA), a mitochondrial enzyme encoded by the *CA5A* gene. As part of the carbonic anhydrase family, CA5A catalyzes the reversible hydration of carbon dioxide to bicarbonate and protons, playing a critical role in pH homeostasis, metabolic pathways, and mitochondrial function. CA5A is highly expressed in metabolically active tissues, such as the liver, kidney, and pancreas, where it supports urea synthesis, gluconeogenesis, and energy metabolism. Dysregulation of CA5A has been implicated in metabolic disorders, including hyperammonemia and mitochondrial diseases, due to its role in nitrogen metabolism and mitochondrial bioenergetics.
CA5A antibodies are widely utilized in research to investigate protein expression, localization, and functional interactions. They are essential tools in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study CA5A's tissue distribution and alterations in disease models. Recent studies also explore CA5A's potential as a biomarker in cancer, as abnormal expression correlates with tumor progression and metabolic reprogramming. Commercial CA5A antibodies are typically validated for specificity using knockout controls or siRNA-based silencing. Researchers prioritize antibodies with high affinity and minimal cross-reactivity to other CA isoforms (e.g., CA-II or CA-IX) to ensure accurate experimental outcomes. Continued interest in CA5A stems from its therapeutic potential in targeting metabolic vulnerabilities in cancer and genetic disorders.
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