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Human Folate receptor alpha(FOLR1) ELISA kit

  • 中文名： 人叶酸受体α(FOLR1)ELISA kit
货号: IPD-EL008784HU
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人叶酸受体α(FOLR1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FOLR1含量。FOLR1 即叶酸受体 1，是一种膜结合蛋白，在多种恶性肿瘤中高表达，而在正常组织中低表达或不表达。其研究机制主要聚焦于利用它与叶酸的高亲和力，开发靶向药物、诊断试剂等，以实现肿瘤的精准治疗和早期诊断。试剂盒检测范围为0.39 ng/ml- 25 ng/ml，灵敏度为0.09 ng/ml。适用于探索FOLR1在卵巢癌、肺癌等实体瘤中的表达模式，评估肿瘤微环境中的生物标志物动态变化，或用于叶酸代谢相关药物的体外研究；为研究FOLR1在细胞信号传导、疾病机制及治疗靶点筛选等科研领域提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    adult ELISA Kit; Adult folate binding protein ELISA Kit; Adult folate-binding protein ELISA Kit; FBP ELISA Kit; Folate Binding Protein ELISA Kit; Folate Receptor 1 Adult ELISA Kit; Folate receptor 1 ELISA Kit; Folate Receptor 1 Precursor ELISA Kit; Folate receptor adult ELISA Kit; Folate receptor alpha ELISA Kit; Folate receptor ELISA Kit; FOLR ELISA Kit; FOLR1 ELISA Kit; FOLR1_HUMAN ELISA Kit; FR alpha ELISA Kit; FR-alpha ELISA Kit; FRalpha ELISA Kit; KB cells FBP ELISA Kit; MOV18 ELISA Kit; Ovarian cancer associated antigen ELISA Kit; Ovarian tumor associated antigen ELISA Kit; Ovarian tumor associated antigen MOv18 ELISA Kit; Ovarian tumor-associated antigen MOv18 ELISA Kit
  • 缩写：
    FOLR1
  • Uniprot No.：
    P15328
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.39 ng/ml - 25 ng/ml
  • 灵敏度：
    0.09 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Tags & Cell Markers
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human FOLR1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    92


    Range %

    88-97


    1:2

    Average %

    98


    Range %

    91-104


    1:4

    Average %

    90


    Range %

    83-97


    1:8

    Average %

    95


    Range %

    88-102


  • 回收率：

    The recovery of human FOLR1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    98

    91-104


    EDTA plasma (n=4)

    93

    88-97


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    25

    2.715

    2.634

    2.674

    2.553


    12.5

    2.072

    2.167

    2.119

    1.998


    6.25

    1.360

    1.328

    1.344

    1.223


    3.12

    0.821

    0.839

    0.830

    0.709


    1.56

    0.477

    0.491

    0.484

    0.363


    0.78

    0.293

    0.303

    0.298

    0.177


    0.39

    0.192

    0.184

    0.188

    0.067


    0

    0.117

    0.125

    0.121




  • 货期：
    3-5 working days

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