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产品描述：人叉头框蛋白03(FoxO3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的FOXO3含量。FOXO3是一种转录因子，属于叉头家族，具有独特的叉头结构域。它通过调节细胞周期、促进凋亡、抗氧化应激损伤等生物学过程，在细胞存活和衰老中发挥重要作用。研究显示，FOXO3在保护神经干细胞、调节氧化应激反应和维持细胞稳态中具有关键作用，与人类长寿和健康密切相关。试剂盒检测范围为1.56 pg/mL-100 pg/mL，适用于细胞生物学研究、疾病模型建立、药物干预效果评估等科研场景，特别适合需要检测低丰度FoxO3表达的体外实验，如干细胞分化、衰老机制探究或抗氧化药物开发等研究领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AF6q21 ELISA Kit; AF6q21 protein ELISA Kit; DKFZp781A0677 ELISA Kit; FKHR2 ELISA Kit; FKHRL 1 ELISA Kit; FKHRL1 ELISA Kit; FKHRL1P2 ELISA Kit; Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 ELISA Kit; Forkhead box O3 ELISA Kit; Forkhead box O3A ELISA Kit; Forkhead box protein O3 ELISA Kit; Forkhead box protein O3A ELISA Kit; Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 ELISA Kit; Forkhead homolog (rhabdomyosarcoma) like 1 ELISA Kit; Forkhead in rhabdomyosarcoma like 1 ELISA Kit; Forkhead in rhabdomyosarcoma-like 1 ELISA Kit; FOX O3A ELISA Kit; FOXO2 ELISA Kit; foxo3 ELISA Kit; FOXO3_HUMAN ELISA Kit; FOXO3A ELISA Kit; MGC12739 ELISA Kit; MGC31925 ELISA Kit
缩写：FOXO3
Uniprot No.：O43524
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FOXO3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 87 | ||||
| Range % | 80-92 | |||||
| 1:200 | Average % | 99 | ||||
| Range % | 91-105 | |||||
| 1:400 | Average % | 101 | ||||
| Range % | 92-110 | |||||
| 1:800 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human FOXO3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-98 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected1002.9432.9032.9232.818502.4172.3482.3832.278251.6851.6631.6741.56912.51.0750.9891.0320.9276.250.5970.6030.6000.4953.120.3740.3570.3660.2611.560.2360.2290.2330.12800.1090.1010.105 | ||||||
货期：3-5 working days
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