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产品描述：山羊促卵泡素(FSH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的FSHB含量。FSHB（促卵泡激素β亚基）是一种由人类基因FSHB编码的蛋白质，位于垂体前叶，对生殖功能至关重要。FSHB与FSHα亚基结合，形成促卵泡激素（FSH），调节性腺活动，包括卵泡发育和精子生成。研究显示，FSHB基因突变可能导致生殖能力下降。试剂盒检测范围为20 mIU/mL-800 mIU/mL，适用于科研领域中对山羊繁殖生理机制、生殖疾病模型构建、畜牧业繁殖力优化等研究，例如发情周期动态监测、卵巢功能评估、激素干预效果分析等场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FSHBFollitropin subunit beta ELISA kit; Follicle-stimulating hormone beta subunit ELISA kit; FSH-B ELISA kit; FSH-beta ELISA kit; Follitropin beta chain ELISA kit
缩写：FSHB
Uniprot No.：Q8HY83
种属：Capra hircus (Goat)
样本类型：serum, plasma
检测范围：20 mIU/mL-800 mIU/mL
灵敏度：20 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of goat FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 87-108 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 82-98 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 85-105 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 84-100 | |||||
回收率：
| The recovery of goat FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-96 | ||||
| EDTA plasma (n=4) | 92 | 87-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2Average 8000.1380.1320.135 4000.2260.2410.234 1840.3430.3200.332 600.6100.5880.599 201.2411.2151.228 02.6382.6072.623 | ||||||
货期：3-5 working days
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