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产品描述：犬游离甲状腺素(FT4)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FT4含量。FT4即游离甲状腺素，是甲状腺功能检查的重要靶点。它能直接反映甲状腺功能状态。研究机制上，主要通过检测其在血液中的含量，辅助医生判断甲状腺疾病，如甲亢时FT4水平升高，甲减时则降低。试剂盒检测范围为7.5 ng/mL-120 ng/mL，适用于科研领域中对犬类甲状腺功能的机制研究、疾病模型评估、营养代谢实验或药理学研究，能够为动物医学、比较内分泌学等基础研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：FT4
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：7.5 ng/mL-120 ng/mL
灵敏度：7.5 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<15% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of canine FT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 90 | |||||
| Range % | 83-97 | ||||||
| 1:2 | Average % | 90 | |||||
| Range % | 88-100 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 90-105 | ||||||
| 1:8 | Average % | 91 | |||||
| Range % | 85-102 | ||||||
回收率：
| The recovery of canine FT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 87-105 | |||||
| EDTA plasma (n=4) | 95 | 89-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2Average 1200.1510.1480.150 600.2800.2830.282 300.4550.4620.459 150.8690.8610.865 7.51.8231.8601.842 | |||||||
货期：3-5 working days
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