首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人游离甲状腺素(FT4)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的FT4含量。FT4即游离甲状腺素，是甲状腺功能检查的重要指标。它能反映甲状腺的功能状态，不受甲状腺结合蛋白影响。其研究机制主要围绕甲状腺激素的合成、分泌、调节等过程，对诊断甲亢、甲减等甲状腺疾病具有关键意义。试剂盒检测范围为4 pmol/L-64 pmol/L，该产品可应用于科研领域中对甲状腺功能调控机制的研究，例如甲状腺功能亢进/减退动物模型评估、环境因素对甲状腺激素代谢的影响分析，或药物干预实验中激素水平变化的动态监测，为内分泌系统相关基础研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：FT4
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：4 pmol/L-64 pmol/L
灵敏度：1.08 pmol/L
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<15% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 85-99 | ||||||
| 1:2 | Average % | 90 | |||||
| Range % | 87-100 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 88-98 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 86-98 | ||||||
回收率：
| The recovery of human FT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 91-98 | |||||
| EDTA plasma (n=4) | 96 | 93-102 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pmol/LOD1OD2Average 42.5472.6462.597 81.4351.4951.465 160.7970.8510.824 320.5170.5230.520 640.3070.2950.301 | |||||||
货期：3-5 working days
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