首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人铁蛋白重链（FTH）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的FTH1含量。FTH1是一种铁结合蛋白，主要功能是储存铁离子。在研究过程中，FTH1被作为靶点，用于开发或筛选缓解肺纤维化的药物。研究发现，FTH1通过调控铁代谢和谷胱甘肽代谢，抑制铁死亡，从而缓解肺纤维化。试剂盒检测范围为0.45 ng/mL-30 ng/mL，可广泛应用于细胞生物学、基础医学等科研领域，支持体外培养细胞铁代谢研究、动物模型组织样本分析以及临床前样本的FTH动态监测，为探究铁超载性疾病、神经退行性病变或肿瘤微环境中铁代谢异常提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Apoferritin ELISA Kit; Cell proliferation inducing gene 15 protein ELISA Kit; Cell proliferation-inducing gene 15 protein ELISA Kit; F HC ELISA Kit; Ferritin H subunit ELISA Kit; Ferritin heavy chain ELISA Kit; Ferritin heavy polypeptide 1 ELISA Kit; FHC ELISA Kit; FRIH ELISA Kit; FRIH_HUMAN ELISA Kit; FTH 1 ELISA Kit; FTH ELISA Kit; FTH1 ELISA Kit; FTH1 protein ELISA Kit; FTHL 6 ELISA Kit; FTHL6 ELISA Kit; Iron overload autosomal dominant ELISA Kit; MGC104426 ELISA Kit; N-terminally processed ELISA Kit; OK/SW-cl.84 ELISA Kit; PIG 15 ELISA Kit; PIG15 ELISA Kit; Placenta immunoregulatory factor ELISA Kit; PLIF ELISA Kit; Proliferation inducing gene 15 protein ELISA Kit; Proliferation inducing protein 15 ELISA Kit
缩写：FTH1
Uniprot No.：P02794
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.45 ng/mL-30 ng/mL
灵敏度：0.11 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 85-103 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 87-106 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 85-100 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-105 | |||||
回收率：
| The recovery of human FTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 83-105 | ||||
| EDTA plasma (n=4) | 96 | 88-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 302.7122.7202.7162.594 151.9191.8511.8851.763 7.51.1661.1241.1451.023 3.750.7250.7370.7310.609 1.80.4060.4280.4170.295 0.90.2820.3010.2920.170 0.450.1930.2020.1980.076 00.1200.1230.122 | ||||||
货期：3-5 working days
×
