| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| WB Predicted band size | 43 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human CDKL4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CDKL4抗体的3篇参考文献示例，基于假设性研究整理：
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1. **文献名称**：*Characterization of a Novel Anti-CDKL4 Antibody for Detecting Cyclin-Dependent Kinase-Like 4 in Breast Cancer*
**作者**：Smith J et al.
**摘要**：本研究开发了一种兔源单克隆抗体，用于特异性检测CDKL4蛋白在乳腺癌组织中的表达。通过Western blot和免疫组化验证，发现CDKL4的高表达与患者预后不良显著相关，提示其可能作为乳腺癌的潜在生物标志物。
2. **文献名称**：*CDKL4 Interacts with p21 to Regulate Cell Cycle Progression*
**作者**：Lee S et al.
**摘要**：利用CDKL4特异性抗体进行免疫共沉淀实验，发现CDKL4与细胞周期调控蛋白p21直接相互作用，影响G1/S期转换。该研究为CDKL4在肿瘤细胞增殖中的作用机制提供了新见解。
3. **文献名称**：*Development of a CDKL4-Specific Monoclonal Antibody for Diagnostic Applications*
**作者**：Gupta R et al.
**摘要**：报道了一种高灵敏度的小鼠单克隆抗体的开发，该抗体通过ELISA和免疫荧光验证，成功应用于多种癌症组织样本的CDKL4检测，显示其在结直肠癌中的表达显著高于正常组织。
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**备注**：若实际文献不足，建议确认“CDKL4”是否为“CDK4”或“CDKL5”的笔误，或检索更新研究。
CDKL4 (Cyclin-Dependent Kinase-Like 4) is a serine/threonine kinase belonging to the CDK family, which plays roles in cell cycle regulation, transcriptional modulation, and neuronal development. Though less characterized than other CDK members, CDKL4 shares structural homology with CDKL5. a kinase linked to neurodevelopmental disorders like CDKL5 deficiency disorder. CDKL4 is expressed in various tissues, including the brain, and studies suggest potential involvement in cell proliferation, differentiation, and synaptic plasticity. Its exact physiological and pathological functions remain under investigation, with emerging interest in its role in cancers (e.g., glioblastoma, breast cancer) and neurological conditions.
CDKL4 antibodies are essential tools for detecting and studying the protein's expression, localization, and interactions. They enable techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to explore CDKL4 distribution in tissues or cultured cells. Commercial CDKL4 antibodies are typically developed using immunogenic peptide sequences specific to human CDKL4. validated for specificity via knockout controls or siRNA knockdown. Researchers utilize these antibodies to investigate CDKL4's signaling pathways, post-translational modifications, and potential cross-talk with other kinases. Challenges include ensuring antibody selectivity due to homology within the CDK family. Ongoing research aims to clarify CDKL4's biological significance, with antibodies serving as critical reagents for both basic research and therapeutic target validation.
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