首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人游离睾酮(F-TESTO)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的F-TESTO含量。人游离睾酮（F-TESTO）是睾酮的一种游离形式，对男性健康和生理功能至关重要。其研究背景涉及睾酮的生理作用、代谢途径及其与疾病如前列腺癌的关系。研究机制主要关注F-TESTO在体内的生物活性、代谢过程及其对健康的影响。试剂盒检测范围为7.5 pg/mL-1500 pg/mL，适用于精准分析低浓度样本。适用于科研场景下探究内分泌失调、多囊卵巢综合征、代谢综合征等疾病的分子机制，或评估运动生理学、衰老相关激素水平变化等研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：F-TESTO
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：7.5 pg/mL-1500 pg/mL
灵敏度：3.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human F-TESTO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 93-101 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 89-98 | |||||
回收率：
| The recovery of human F-TESTO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 92-104 | ||||
| EDTA plasma (n=4) | 105 | 101-109 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 15000.1740.1760.175 3750.3240.3380.331 1200.5350.5140.525 300.8120.8540.833 7.51.4211.3341.378 01.9871.9121.950 | ||||||
货期：3-5 working days
×
