首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人弗林蛋白酶(FURIN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的FURIN含量。FURIN是一种蛋白质，属于前蛋白转化酶家族。它在多种生理和病理过程中发挥作用，例如参与病毒蛋白加工，与病毒感染性相关。研究发现其能裂解病毒表面糖蛋白，促进病毒入侵宿主细胞，目前是抗病毒等领域潜在研究靶点。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，适用于基础科研中探究FURIN在疾病模型中的表达调控、药物干预效果评估或信号通路研究。例如，可通过检测细胞裂解液中的FURIN水平分析其在病毒感染过程中的激活程度，或利用组织匀浆样本研究肿瘤微环境中的蛋白酶活性变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FURIN; FUR; PACE; PCSK3; Furin; Dibasic-processing enzyme; Paired basic amino acid residue-cleaving enzyme; PACE
缩写：FURIN
Uniprot No.：P09958
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.86 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FURIN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-91 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 94-104 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human FURIN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-100 | ||||
| EDTA plasma (n=4) | 99 | 92-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15001.9251.8521.8891.8057501.5011.4761.4891.4053751.0741.0041.0390.955187.50.7830.7390.7610.677940.4960.4870.4920.408470.3180.3090.3140.23023.50.2330.2290.2310.14700.0870.0810.084 | ||||||
货期：3-5 working days
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