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Human RNA-binding protein FUS (FUS/TLS) ELISA kit

  • 中文名： 人RNA结合蛋白FUS (FUS/TLS) ELISA kit
货号: IPD-E17376h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人RNA结合蛋白FUS (FUS/TLS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、细胞裂解物样本中的FUS含量。FUS是一种重要的靶点。它属于RNA结合蛋白家族，与RNA代谢、转录调控等密切相关。在疾病研究中，FUS的突变或异常表达与神经退行性疾病如肌萎缩侧索硬化症等相关，深入研究其作用机制，有望为相关疾病治疗带来新策略。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于神经退行性疾病模型研究、药物干预后FUS蛋白动态变化分析、细胞应激状态下RNA结合蛋白功能探索等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    75 kDa DNA pairing protein ELISA Kit; 75 kDa DNA-pairing protein ELISA Kit; ALS6 ELISA Kit; Amyotrophic lateral sclerosis 6 ELISA Kit; fus ELISA Kit; FUS CHOP ELISA Kit; Fus like protein ELISA Kit; FUS_HUMAN ELISA Kit; FUS1 ELISA Kit; Fused in sarcoma ELISA Kit; Fusion (involved in t(12,16) in malignant liposarcoma) ELISA Kit; Fusion derived from t(12,16) malignant liposarcoma ELISA Kit; Fusion gene in myxoid liposarcoma ELISA Kit; Heterogeneous nuclear ribonucleoprotein P2 ELISA Kit; hnRNP P2 ELISA Kit; hnRNPP2 ELISA Kit; Oncogene FUS ELISA Kit; Oncogene TLS ELISA Kit; POMp75 ELISA Kit; RNA binding protein FUS ELISA Kit; RNA-binding protein FUS ELISA Kit; TLS ELISA Kit; TLS CHOP ELISA Kit; Translocated in liposarcoma ELISA Kit; Translocated in liposarcoma protein ELISA Kit
  • 缩写：
    FUS
  • Uniprot No.：
    P35637
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell lysates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human FUS/TLS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    100


    Range %

    98-102


    1:2

    Average %

    102


    Range %

    95-108


    1:4

    Average %

    103


    Range %

    99-107


    1:8

    Average %

    93


    Range %

    88-97


  • 回收率：

    The recovery of human FUS/TLS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    90-100


    EDTA plasma (n=4)

    101

    94-108


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    1000

    2.546

    2.634

    2.590

    2.432


    500

    2.001

    1.967

    1.984

    1.826


    250

    1.314

    1.268

    1.291

    1.133


    125

    0.635

    0.629

    0.632

    0.474


    62.5

    0.426

    0.421

    0.424

    0.266


    31.2

    0.285

    0.293

    0.289

    0.131


    15.6

    0.227

    0.214

    0.221

    0.063


    0

    0.161

    0.155

    0.158




  • 货期：
    3-5 working days

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