关键信息
-
基因名
PE-Labeled HLA-A*03:01&B2M Tetramer
- 应用
-
别名
HLA-A*0301 & B2M
-
种属
Human
-
表达系统
HEK293
-
标签
C-Avi;C-His
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P04439-1 (G25-T305)&P61769
-
表达区间
P04439-1 (G25-T305)&P61769 (I21-M119)
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
The PE-Labeled HLA-A*03:01&B2M Tetramer is a significant tool in immunology and cancer research, designed for the identification and characterization of antigen-specific T cells. Human Leukocyte Antigen (HLA) molecules, particularly HLA-A*03:01, play a crucial role in the immune system by presenting peptide antigens to CD8+ T cells, thus eliciting an immune response. The association with Beta-2-Microglobulin (B2M) stabilizes the HLA structure, enhancing its ability to present these peptides effectively. The labeling with phycoerythrin (PE) facilitates fluorescent detection and allows researchers to visualize and quantify T cells that recognize specific peptide-MHC complexes. This technology has profound implications for the study of immune responses in various diseases, including cancer, autoimmune disorders, and infectious diseases. By providing insights into T cell repertoire and functionality, PE-Labeled HLA-A*03:01&B2M Tetramers serve as invaluable tools for developing targeted immunotherapies and understanding T cell-mediated immunity in health and disease. The ongoing advancements in this area focus on optimizing the specificity and sensitivity of tetramer-based assays, which could ultimately lead to improved diagnostics and treatment strategies.
