首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人活化凝血因子X(FXa)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FXa含量。FXa（因子 Xa）是凝血级联反应中的关键酶。其背景在于在凝血过程里发挥核心作用，参与血栓形成。研究机制方面，聚焦于研发针对 FXa 的抑制剂，通过阻断其活性来抑制凝血，进而用于预防和治疗血栓相关疾病，降低血栓风险。试剂盒检测范围为0.312 ng/mL-20 ng/mL，该产品适用于凝血相关分子机制研究、血栓性疾病模型分析、抗凝剂药效评估等科研领域，实验数据稳定可靠，配套提供标准品、试剂及操作指南，满足体外实验需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：FXa
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FXa in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 83-96 | |||||
回收率：
| The recovery of human FXa spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-102 | ||||
| EDTA plasma (n=4) | 96 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.5362.5982.5672.405101.7351.7681.7521.59051.0361.0981.0670.9052.50.6500.6980.6740.5121.250.4460.4320.4390.2770.6250.3420.3580.3500.1880.3120.2400.2610.2510.08900.1610.1630.162 | ||||||
货期：3-5 working days
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