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产品描述：人生长停滞和DNA损伤诱导蛋白GADD45α(GADD45A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的GADD45A含量。GADD45A是一种应激反应蛋白，参与DNA修复、细胞周期调控和衰老等细胞功能。其在肿瘤发生发展中起重要作用，通过调控细胞周期、凋亡、自噬和侵袭转移等过程，影响肿瘤的发生和发展。试剂盒检测范围为25 pg/mL-1600 pg/mL，支持细胞应激机制研究、药物筛选模型建立及疾病相关分子标志物探索等科研用途，为生命科学领域提供可靠的分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：DDIT 1 ELISA Kit; DDIT-1 ELISA Kit; DDIT1 ELISA Kit; DNA damage inducible transcript 1 ELISA Kit; DNA damage-inducible transcript 1 protein ELISA Kit; GA45A_HUMAN ELISA Kit; GADD45 ELISA Kit; GADD45A ELISA Kit; Growth arrest and DNA damage inducible 45 alpha ELISA Kit; Growth arrest and DNA damage inducible alpha ELISA Kit; Growth arrest and DNA damage-inducible protein GADD45 alpha ELISA Kit
缩写：GADD45A
Uniprot No.：P24522
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GADD45A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 89-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 85-90 | |||||
回收率：
| The recovery of human GADD45A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 90-96 | ||||
| EDTA plasma (n=4) | 102 | 99-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected16002.4042.4682.4362.2748001.6211.5381.5801.4184001.0441.0681.0560.8942000.6160.6350.6260.4641000.3960.3720.3840.222500.2890.2750.2820.120250.2180.2150.2170.05500.1600.1630.162 | ||||||
货期：3-5 working days
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