首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠甘丙肽前肽前体(GAL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GAL含量。小鼠甘丙肽前肽前体（GAL）是一种由29个氨基酸组成的神经肽，具有抑制胰岛素分泌和调节突触传导的作用。其通过结合三种G蛋白偶联受体（GalR1~3）发挥作用，其中GalR1和GalR3促进抑郁，而GalR2则具有抗抑郁作用。GAL的研究涉及其在神经系统中的作用，包括对记忆、学习和认知功能的影响。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于小鼠疾病模型构建、神经生物学研究、代谢调控机制探索等科研场景，可稳定检测脑组织、外周循环系统等样本中的GAL动态变化，为探究其在能量代谢、神经信号传导及疼痛调节中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Gal ELISA Kit; Galn ELISA Kit; Galanin peptides [Cleaved into: Galanin; Galanin message-associated peptide ELISA Kit; GMAP)] ELISA Kit
缩写：GAL
Uniprot No.：P47212
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse GAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 96 | |||||
| Range % | 92-104 | ||||||
| 1:2 | Average % | 93 | |||||
| Range % | 86-98 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 87-95 | ||||||
| 1:8 | Average % | 102 | |||||
| Range % | 96-107 | ||||||
回收率：
| The recovery of mouse GAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 88-95 | |||||
| EDTA plasma (n=4) | 107 | 101-112 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20002.6212.7142.6682.55810002.2032.3202.2622.1525001.6551.6991.6771.5672501.0471.1221.0850.9751250.7880.7450.7670.65762.50.4630.4620.4630.35331.250.2120.2230.2180.10800.1080.1110.110 | |||||||
货期：3-5 working days
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