| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CSS1; OSA2; 6A3-5; DAN15; MRD12; P250R; BRIGHT; BAF250B; ELD/OSA1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human ARID1B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇关于ARID1B抗体的代表性文献(模拟数据供参考，实际文献需通过数据库检索获取)：
1. **文献名称**: "ARID1B as a epigenetic regulator in neurodevelopmental disorders"
**作者**: Smith J. et al. (2020)
**摘要**: 研究使用ARID1B抗体(货号AB123)通过染色质免疫沉淀测序(ChIP-seq)揭示ARID1B蛋白在神经细胞分化中调控靶基因的机制，发现其缺失导致组蛋白修饰异常。
2. **文献名称**: "Validation of ARID1B-specific antibodies for clinical diagnostics"
**作者**: Lee H. et al. (2019)
**摘要**: 对比多种市售ARID1B抗体(包括Sigma-Aldrich #A5678和Abcam ab12345)，通过Western blot和免疫组化验证抗体特异性，发现部分抗体存在交叉反应，提出标准化检测流程。
3. **文献名称**: "ARID1B loss promotes tumorigenesis in colorectal cancer"
**作者**: Wang X. et al. (2021)
**摘要**: 利用ARID1B敲除小鼠模型及抗体免疫荧光染色，证明ARID1B蛋白表达缺失通过Wnt信号通路激活促进结直肠癌发展，提示其作为潜在生物标志物。
(注：以上为模拟示例，实际引用需检索PubMed/Google Scholar，关键词：ARID1B antibody, chromatin remodeling, Coffin-Siris syndrome)
The ARID1B (AT-rich interaction domain 1B) antibody is a tool used to detect the ARID1B protein, a critical subunit of the SWI/SNF chromatin remodeling complex. This complex regulates gene expression by altering chromatin structure, enabling access to transcription factors. ARID1B specifically recognizes DNA sequences rich in AT base pairs, guiding SWI/SNF to target genomic regions. Mutations in ARID1B are linked to neurodevelopmental disorders like Coffin-Siris syndrome, autism, and intellectual disability, as well as cancers such as ovarian, gastric, and lung carcinomas. The antibody aids in studying ARID1B's role in cellular differentiation, tumor suppression, and epigenetic regulation.
ARID1B antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunoprecipitation (IP) to assess protein expression, localization, and interactions. Due to high homology among ARID family members, antibody specificity is crucial to avoid cross-reactivity. Some antibodies target unique regions of ARID1B, such as its C-terminal domain, to ensure accuracy. Commercial ARID1B antibodies are often validated in knockout cell lines to confirm specificity.
Research using these antibodies has revealed ARID1B's dual role as both an oncogene and tumor suppressor, depending on cellular context. Its loss in cancers often correlates with poor prognosis, while haploinsufficiency in developmental disorders underscores its importance in neural and tissue maturation. ARID1B antibodies remain vital for exploring molecular mechanisms in disease and therapeutic targeting.
×
