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产品描述：人生长停滞特异性蛋白6(GAS6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GAS6含量。GAS6是一种生长停滞特异性蛋白，在细胞生长、存活和迁移等过程中发挥关键作用。其研究机制主要围绕与受体酪氨酸激酶结合，激活下游信号通路。深入研究GAS6有助于了解相关疾病发病机制，为开发靶向治疗策略提供理论基础。试剂盒检测范围为0.78 ng/mL-50 ng/mL，适用于基础科研中疾病分子机制研究、药物靶点筛选验证、生物标志物探索等场景，尤其可为肿瘤免疫微环境、血管生成调控及炎症相关疾病的分子病理研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AXL receptor tyrosine kinase ligand ELISA Kit; AXL stimulatory factor ELISA Kit; AXLLG ELISA Kit; AXS F ELISA Kit; AXSF ELISA Kit; DKFZp666G247 ELISA Kit; FLJ34709 ELISA Kit; Gas 6 ELISA Kit; GAS-6 ELISA Kit; Gas6 ELISA Kit; GAS6_HUMAN ELISA Kit; Growth arrest specific 6 ELISA Kit; Growth arrest-specific protein 6 ELISA Kit; Growth-arrest-specific protein 6 ELISA Kit; Growth-arrest-specific protein 6 precursor ELISA Kit
缩写：GAS6
Uniprot No.：Q14393
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GAS6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 89-96 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 84-92 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 93-102 | |||||
回收率：
| The recovery of human GAS6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 92-97 | ||||
| EDTA plasma (n=4) | 96 | 92-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected502.3882.3522.3702.226251.6621.5761.6191.47512.51.0611.0421.0520.9086.250.6140.6000.6070.4633.120.4090.4170.4130.2691.560.3030.3090.3060.1620.780.2380.2410.2400.09600.1420.1460.144 | ||||||
货期：3-5 working days
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