首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠胃泌素(Gastrin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液,组织匀浆样本中的GAST含量。GAST作为一种靶点，其背景涉及在生理过程中发挥重要作用，如调节胃肠道功能等。在研究机制方面，主要聚焦于其信号传导通路，探索其与疾病发生、发展的关联，旨在通过干预该靶点来开发针对相关疾病的治疗策略和药物。试剂盒检测范围为0.312 pg/mL-20 pg/mL，该产品适用于胃肠功能研究、药物对胃泌素分泌影响的评价及消化系统疾病模型中激素水平动态分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Gast ELISA Kit; Gas ELISA Kit; Gastrin [Cleaved into: Big gastrin ELISA Kit; Gastrin-34 ELISA Kit; G34); Gastrin] ELISA Kit
缩写：GAST
Uniprot No.：P04563
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates ,tissue homogenates
检测范围：0.312 pg/mL-20 pg/mL
灵敏度：0.078 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat gastrin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:200 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:400 | Average % | 99 | ||||
| Range % | 95-104 | |||||
| 1:800 | Average % | 102 | ||||
| Range % | 98-107 | |||||
回收率：
| The recovery of rat gastrin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-96 | ||||
| EDTA plasma (n=4) | 100 | 95-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected202.4962.5372.5172.390102.1812.1922.1872.06051.7951.7411.7681.6412.51.3531.3131.3331.2061.250.8780.8980.8880.7610.6250.3690.3660.3680.2410.3120.2420.2590.2510.12400.1230.1300.127 | ||||||
货期：3-5 working days
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