首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人葡萄糖神经酰胺酶(GBA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GBA含量。GBA（葡萄糖脑苷脂酶）是一种溶酶体酶，其突变会导致戈谢病。GBA在帕金森病中也扮演重要角色，其突变可能干扰α-突触核蛋白的降解，引发神经退行性疾病。研究GBA机制有助于发现新的治疗靶点和干预策略。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于细胞代谢研究、疾病模型构建、药物干预效果评估等科研场景，为神经科学、代谢疾病研究等领域提供可靠的定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Acid beta glucosidase ELISA Kit; Acid beta-glucosidase ELISA Kit; Alglucerase ELISA Kit; Beta glucocerebrosidase ELISA Kit; BETA GLUCOSIDASE, ACID ELISA Kit; Beta-glucocerebrosidase ELISA Kit; betaGC ELISA Kit; D glucosyl N acylsphingosine glucohydrolase ELISA Kit; D-glucosyl-N-acylsphingosine glucohydrolase ELISA Kit; EC 3.2.1.45 ELISA Kit; GBA ELISA Kit; Gba protein ELISA Kit; GBA1 ELISA Kit; GC ELISA Kit; GCase ELISA Kit; GCB ELISA Kit; GLCM_HUMAN ELISA Kit; GLUC ELISA Kit; Glucocerebrosidase (alt.) ELISA Kit; Glucocerebrosidase ELISA Kit; GLUCOCEREBROSIDASE PSEUDOGENE ELISA Kit; Glucosidase beta ELISA Kit; Glucosidase, beta, acid ELISA Kit; Glucosidase, beta, acid (includes glucosylceramidase) ELISA Kit; Glucosylceramidase ELISA Kit; Imiglucerase ELISA Kit; Lysosomal glucocerebrosidase ELISA Kit; OTTHUMP00000033992 ELISA Kit; OTTHUMP00000033993 ELISA Kit
缩写：GBA
Uniprot No.：P04062
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GBA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 89-100 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-98 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 83-93 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 88-96 | |||||
回收率：
| The recovery of human GBA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-103 | ||||
| EDTA plasma (n=4) | 88 | 84-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.4432.5682.5062.412102.1262.1692.1482.05451.6531.6981.6761.5822.51.2211.3051.2631.1691.250.7390.7460.7430.6490.6250.3800.4100.3950.3010.3120.2110.2090.2100.11600.0960.0910.094 | ||||||
货期：3-5 working days
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