| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/500-1/1000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human ATG2A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4条关于ATG2A抗体的参考文献示例(虚拟内容，仅供格式参考)：
1. **文献名称**：Structural insights into ATG2A-mediated lipid transfer in autophagy.
**作者**：Valente G, et al.
**摘要**：本研究解析了ATG2A蛋白的三维结构，揭示了其介导脂质转移的分子机制，并开发了特异性抗体用于检测其在自噬体膜形成中的动态定位。
2. **文献名称**：ATG2A deficiency disrupts autophagosome-lysosome fusion and promotes tumorigenesis.
**作者**：Tamura N, et al.
**摘要**：通过ATG2A基因敲除模型和特异性抗体检测，发现ATG2A缺失导致自噬流阻断，促进结直肠癌细胞增殖，提示其抑癌作用。
3. **文献名称**：Antibody-based profiling of ATG2A in neurodegenerative diseases.
**作者**：Maruyama T, Noda T.
**摘要**：利用高特异性ATG2A抗体，发现帕金森病患者脑组织中ATG2A表达异常，与自噬功能障碍和α-synuclein聚集相关。
4. **文献名称**：Development of a monoclonal antibody targeting human ATG2A for functional studies.
**作者**：Kishi-Itakura C, et al.
**摘要**：报道了一种新型ATG2A单克隆抗体的制备与验证，证明其在免疫印迹、免疫荧光及Co-IP实验中的高效性和特异性。
(注：上述文献为虚拟示例，实际文献需通过PubMed或学术数据库检索。)
ATG2A (Autophagy-related protein 2A) is a key regulator of autophagy, a cellular degradation process essential for maintaining homeostasis. This protein plays a critical role in autophagosome formation by mediating lipid transfer from the endoplasmic reticulum to expanding phagophore membranes, enabling membrane elongation and closure. ATG2A interacts with WIPI proteins and LC3-II, forming a bridge between donor and acceptor membranes during autophagosome biogenesis. Its dysfunction is linked to impaired autophagy, contributing to pathologies like cancer, neurodegenerative diseases, and metabolic disorders.
ATG2A antibodies are tools used to study the expression, localization, and function of ATG2A in research. They are commonly employed in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to investigate autophagy dynamics. These antibodies help identify ATG2A's interaction partners and its role in stress responses or disease models. Specificity validation is crucial, as ATG2A shares homology with its paralog ATG2B. Studies using ATG2A antibodies have revealed its phosphorylation status during nutrient deprivation and its involvement in selective autophagy pathways. Recent research also explores ATG2A's lipid transfer mechanism and its potential as a therapeutic target for autophagy-related disorders.
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