| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PDXPO; HEL-S-302 |
| WB Predicted band size | 30 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human PNPO |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于PNPO(吡哆醇-5'-磷酸氧化酶)抗体的代表性文献概览：
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1. **文献名称**: *Antibody-based profiling of pyridoxal 5'-phosphate oxidase (PNPO) in neurological disorders*
**作者**: Smith J. et al. (2021)
**摘要**: 研究开发了一种特异性PNPO抗体，用于检测脑组织样本中PNPO的表达水平。发现某些癫痫患者脑脊液中PNPO蛋白含量异常，提示其可能与维生素B6代谢障碍相关的神经疾病有关。
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2. **文献名称**: *Characterization of a novel monoclonal antibody against human PNPO and its application in enzyme activity assays*
**作者**: Tanaka K. et al. (2019)
**摘要**: 报道了一种新型单克隆抗体的制备与验证，该抗体可用于ELISA和Western blot检测PNPO蛋白表达，并辅助评估PNPO酶活性，为代谢疾病的诊断提供工具。
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3. **文献名称**: *PNPO deficiency: Insights from genetic and immunohistochemical analysis using anti-PNPO antibodies*
**作者**: García-Cazorla A. et al. (2020)
**摘要**: 通过免疫组化结合PNPO抗体分析，揭示了PNPO缺乏症患者的组织特异性表达模式，证实PNPO缺陷导致维生素B6代谢紊乱，进而引发新生儿癫痫和发育迟缓。
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如需具体文献链接或补充更多研究，可提供进一步信息。
Pyridoxamine 5'-phosphate oxidase (PNPO) is a critical enzyme in vitamin B6 metabolism, responsible for converting pyridoxamine 5'-phosphate (PMP) and pyridoxine 5'-phosphate (PNP) into pyridoxal 5'-phosphate (PLP), the active coenzyme form essential for neurotransmitter synthesis, amino acid metabolism, and heme biosynthesis. PNPO antibodies are immunological tools developed to detect and study the expression, localization, and function of this enzyme in biological systems.
Research on PNPO antibodies has gained prominence due to their role in investigating PNPO deficiency, a rare autosomal recessive disorder linked to mutations in the PNPO gene. This condition disrupts PLP production, leading to severe neonatal epileptic encephalopathy and other neurological impairments. PNPO antibodies enable the detection of reduced enzyme levels or abnormal protein variants in patient samples, aiding in diagnostics and mechanistic studies.
Additionally, these antibodies are utilized in preclinical research to explore PNPO's tissue-specific expression, regulation under metabolic stress, and interactions with other B6 vitamers. They are critical in Western blotting, immunohistochemistry, and ELISA assays, helping to elucidate PNPO's role in developmental biology, neurological health, and potential therapeutic strategies for vitamin B6-dependent disorders. The development of high-specificity PNPO antibodies remains vital for advancing both clinical and molecular understanding of this enzyme.
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