| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CEMCOX2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human COX15 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于COX15抗体的3篇参考文献及其摘要概括：
1. **"Mutations in COX15 produce a defect in the mitochondrial heme biosynthetic pathway, causing early-onset fatal hypertrophic cardiomyopathy"**
- **作者**: Antonicka, H., et al.
- **摘要**: 本研究通过Western blot和免疫荧光技术，利用COX15抗体分析患者细胞系中COX15蛋白表达及线粒体定位，揭示了COX15基因突变导致血红素合成缺陷，进而引发早发性心肌病。
2. **"COX15 mutations associated with inherited retinal dystrophy and cardiomyopathy"**
- **作者**: Horváth, R., et al.
- **摘要**: 通过免疫组化和Western blot，研究者使用COX15抗体检测患者视网膜及心肌组织中的蛋白表达缺失，发现COX15突变导致多系统线粒体功能障碍，表现为视网膜病变和心脏异常。
3. **"Mitochondrial protein COX15 interacts with DNA repair enzyme HMG-CoA reductase degradation protein 1 (HRD1)"**
- **作者**: Bai, Y., et al.
- **摘要**: 该研究通过免疫共沉淀(Co-IP)和免疫印迹技术，利用COX15抗体验证其与HRD1的相互作用，提示COX15可能通过线粒体-内质网关联参与细胞应激反应。
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**说明**：
- 上述文献均涉及使用COX15抗体进行蛋白表达分析(如Western blot、免疫荧光/组化)或蛋白互作研究，涵盖疾病机制(心肌病、视网膜病变)及分子功能探索。
- 若需具体文献来源(期刊、年份)，可进一步补充数据库检索信息(如PubMed ID)。
The COX15 antibody is a research tool used to study the COX15 protein, a key component in mitochondrial energy production. COX15. or cytochrome c oxidase assembly protein homolog 15. is essential for the biogenesis of cytochrome c oxidase (Complex IV), the terminal enzyme in the mitochondrial electron transport chain (ETC). It catalyzes the synthesis of heme \(a\), a prosthetic group critical for Complex IV activity. Mutations in the COX15 gene are linked to mitochondrial disorders, such as Leigh syndrome and hypertrophic cardiomyopathy, characterized by impaired cellular energy metabolism.
COX15 antibodies enable the detection and quantification of COX15 protein levels in various experimental models, including cell cultures, tissues, and animal samples. These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate COX15 expression, subcellular localization, and interactions. Researchers employ COX15 antibodies to explore mitochondrial dysfunction mechanisms, assess disease biomarkers, and evaluate therapeutic interventions targeting ETC defects. Their specificity and validation are crucial for ensuring accurate results in studies on mitochondrial biology, metabolic diseases, and aging-related pathologies.
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