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产品描述：猪胰高血糖素(GC)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GCG含量。GCG 即胰高血糖素基因，其编码的胰高血糖素在血糖调节中作用关键，与胰岛素共同维持血糖稳态。研究机制方面，聚焦于其信号通路，通过与受体结合发挥功能，还用于研发调控血糖的药物，在糖尿病等代谢疾病治疗上有重要研究价值。试剂盒检测范围为125 pg/mL-2000 pg/mL，该产品可广泛应用于动物营养学、代谢性疾病模型研究、激素调控机制探索等科研领域，例如通过检测不同饲喂条件或疾病状态下猪的GC动态变化，为糖代谢研究、药效评价及生理调控提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GCGGlucagon [Cleaved into: Glicentin; Glicentin-related polypeptide ELISA Kit; GRPP); Oxyntomodulin ELISA Kit; OXM ELISA Kit; OXY); Glucagon; Glucagon-like peptide 1 ELISA Kit; GLP-1); Glucagon-like peptide 1(7-37 ELISA Kit; GLP-1(7-37)); Glucagon-like peptide 1(7-36 ELISA Kit; GLP-1(7-36)); Glucagon-like peptide 2 ELISA Kit; GLP-2)] ELISA Kit
缩写：GCG
Uniprot No.：P01274
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：125 pg/mL-2000 pg/mL
灵敏度：62.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig GC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 85-96 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 92-102 | |||||
| 1:4 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-96 | |||||
回收率：
| The recovery of pig GC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 87-97 | ||||
| EDTA plasma (n=4) | 96 | 90-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average20000.1200.1110.11610000.3150.3400.3285000.6010.6110.6062501.0180.9450.9821252.0361.9231.980 | ||||||
货期：3-5 working days
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