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产品描述：人骨形态发生蛋白3B(GDF10)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GDF10含量。GDF10（生长分化因子10）是一种TGF-β超家族成员，参与多种生物学过程，如骨骼形态发生、神经修复和肿瘤抑制。其作用机制涉及与TGF-β受体结合，激活下游信号通路，调节基因表达。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，该产品可广泛应用于骨骼发育机制研究、纤维化疾病模型分析、药物干预效果评价等科研领域，为探索GDF10在组织再生及病理调控中的分子机制提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BIP ELISA Kit; BMP 3b ELISA Kit; BMP-3b ELISA Kit; BMP3B ELISA Kit; BMP3B_HUMAN ELISA Kit; Bone inducing protein ELISA Kit; Bone morphogenetic protein 3b [Precursor] ELISA Kit; Bone morphogenetic protein 3b ELISA Kit; Bone-inducing protein ELISA Kit; GDF 10 ELISA Kit; GDF-10 ELISA Kit; Gdf10 ELISA Kit; growth differentiation factor 10 ELISA Kit; Growth/differentiation factor 10 ELISA Kit
缩写：GDF10
Uniprot No.：P55107
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GDF10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 107 | |||||
| Range % | 104-110 | ||||||
| 1:2 | Average % | 103 | |||||
| Range % | 101-105 | ||||||
| 1:4 | Average % | 88 | |||||
| Range % | 84-94 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 91-97 | ||||||
回收率：
| The recovery of human GDF10 piked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 91-103 | |||||
| EDTA plasma (n=4) | 87 | 85-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 15001.9562.1212.0391.906 7501.5311.5471.5391.406 3751.1011.1121.1070.974 187.50.6700.7150.6930.560 940.4240.4570.4410.308 470.3010.3220.3120.179 23.50.2210.2350.2280.095 00.1300.1360.133 | |||||||
货期：3-5 working days
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