Mouse Growth/differentiation factor 15(GDF15) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-EL009345MO

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠生长分化因子15(GDF15)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GDF15含量。GDF15属于TGF-β超家族成员，在细胞应激时表达上调。其作为应激反应细胞因子，能调节能量代谢等生理过程。研究聚焦于它在代谢性疾病（如肥胖、糖尿病）及癌症等领域的作用机制，有望为相关疾病诊疗提供新思路。试剂盒检测范围为7.8 pg/ml-500 pg/ml，支持科研人员探究GDF15在代谢综合征、肥胖相关疾病、心血管异常及肿瘤微环境调控等领域的分子机制，为动物模型研究提供可靠工具；适用于实验室常规检测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Gdf15 ELISA Kit; Mic1 ELISA Kit; SbfGrowth/differentiation factor 15 ELISA Kit; GDF-15 ELISA Kit; Macrophage inhibitory cytokine 1 ELISA Kit; MIC-1 ELISA Kit
缩写：
GDF15
Uniprot No.：
Q9Z0J7
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
7.8 pg/ml -500 pg/ml
灵敏度：
1.95 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Signal Transduction
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse GDF15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	95
	Range %	91-99
1:4	Average %	92
	Range %	87-98
1:8	Average %	93
	Range %	88-96

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
500	2.254	2.111	2.183	2.020
250	1.636	1.694	1.665	1.502
125	1.022	1.112	1.067	0.904
62.5	0.575	0.603	0.589	0.426
31.2	0.334	0.337	0.336	0.173
15.6	0.228	0.237	0.233	0.070
7.8	0.201	0.215	0.208	0.045
0	0.160	0.166	0.163